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MLP/MTB assay

[multilineage progenitor assay; M stands for myeloid cells; T stands for T-cells, B stands for B-cells] This assay has been described originally by Kawamoto et al (1997) as MLP assay. The culture system involves fetal thymic organ culture because development of T-cells is highly dependent on thymic architecture. In this system hematopoietic progenitor cells are co-cultured with deoxyguanosine-treated fetal thymus lobe in the presence of IL7, IL3 and SCF. The progenitor cell types then populate the fetal thymus lobe and can be assayed by flow cytometry for the expression of markers characteristic of T-cells, B-cell, or ... ... ... ... ... Subscribe to continue reading! ... ...

 

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