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conditioned medium

[conditioned media, culture supernatant, culture supernatants]

abbr. CM. Occasionally referred to also as CFCS (cell-free culture supernatant). CM is the general term to describe media in which cells have been cultivated already for a period of time. The medium is obtained by sterile filtration of used culture media and is added to fresh culture media for up to one third of the final volume.

Conditioned media are used for the cultivation of particularly fastidious cells and cell lines (see, for example: ES cells) because they contain many mediator substances that were secreted by the cells cultivated in this medium. These mediator substances contain growth factors and ... ... ... ... ... Subscribe to continue reading! ... ...

 

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