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cDNA

abbr. for complementary DNA, the single-stranded or double-stranded DNA copy of a mRNA. cDNA is obtained by a number of successive enzymatic reactions in vitro that are known as reverse transcription (see also: gene expression). The starting material for the synthesis of cDNA can be the entire cellular or cytoplasmic RNA but also mRNA purified by affinity chromatography. The most important enzyme for the synthesis of cDNA are RNA-dependent DNA polymerases known as reverse transcriptases. These enzymes are capable of utilizing deoxyribonucleoside triphosphates as substrate and RNA as a template for the synthesis of a DNA strand. The primary reaction product of a reverse transcription reaction is a hybrid double-stranded molecule consisting of the parent RNA strand and the newly synthesized DNA ... ... ... ... ... Subscribe to continue reading! ... ...

 

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