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RAW264.7

a murine macrophage-like cell line. An assay for IFN-gamma, which measures the concentration of nitric oxide (determined by nitrite accumulation in the culture medium) generated by RAW264.7 cells stimulated with IFN-gamma has been developed. Following the incubation of cells with murine IFN-gamma, NO production is found to be linear at IFN-gamma concentrations between 0 and 10 U/mL and logarithmically linear between 2 and 100 U/mL (Kim and Son, 1996).

The cells constitutively produce VEGF (Xiong et al, 1998). VEGF production can be increased further under hypoxic conditions or after ... ... ... ... ... Subscribe to continue reading! ... ...

 

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