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MTT assay

This test is a quantitative colorimetric method to determine cell proliferation. It utilizes the yellow tetrazolium salt [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide] which is metabolized by mitochondrial succinic dehydrogenase activity of proliferating cells to yield a purple formazan reaction product.


formula of MTT


Cells do not require functional mitochondria as demonstrated by the observation that no differences are observed in formazan production by normal cells or respiratory-defective cells ... ... ... ... ... Subscribe to continue reading! ... ...

 

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