MTT |
MtTF1 |
HHT1 |
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This test is a quantitative colorimetric method to determine cell proliferation. It utilizes the yellow tetrazolium salt [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide] which is metabolized by mitochondrial succinic dehydrogenase activity of proliferating cells to yield a purple formazan reaction product.
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Cells do not require functional mitochondria as demonstrated by the observation that no differences are observed in formazan production by normal cells or respiratory-defective cells
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