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L929

This murine aneuploid fibrosarcoma cell line is used to assay TNF-alpha and TNF-beta (see also: bioassays, cytokine assays), which lyse cells sensitized by pretreatment with actinomycin or cycloheximide. Treatment with TNF initiates apoptosis and subsequent cell death. The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164 (see: WEHI-3B). Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF ... ... ... ... ... Subscribe to continue reading! ... ...

 

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