MAPPIT

[mammalian protein-protein interaction trap] This procedure permits the analysis of protein-protein interactions under physiological conditions in mammalian cells. It can be employed to study known protein-protein interactions by targeted mutagenesis, or to screen for novel interactions.

The system as described in the original publication employs a mutated leptin receptor and the use of a modified signaling transducer protein, gp130, but it can be used with other receptor constructs carrying different extracellular ligand binding domains.

The basis of the interaction strategy is as follows: binding of a ligand to a type 1 cytokine receptor ... ... ... ...
 
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Entry last modified: January 2002