Rat Insulinoma Gene |
rat neural antigen-2 |
non-cell-autonomous necrosis |
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Rat liver 13k protein (115 amino acids) has glutathione binding activity. The nucleotide sequence of its cDNA predicts a protein with 99.1 %, 89.6 % and 73.0 % homology with mouse, human and chicken macrophage migration inhibitory factor (MIF), respectively, indicating that the rat liver 13k protein and MIF are homologous.
The N-terminal 25 amino acids show 35 % sequence similarity with that of the rat glutathione transferase Yb subunit. Northern blot analysis indicates that this gene is expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney. Southern blot analysis suggests that the MIF gene has many pseudo-genes or/and closely related genes.
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