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hepatospheres

[hepatosphere]

This term has been used to refer to multicellular aggregates obtained from hepatocytes in three-dimensional culture or by coculturing hepatocytes withnon-parenchymal liver cells such as hepatic stellate cells (Gody et al, 2013; van Zijl and Mikulits, 2010). The term is used also for aggregates of hepatic-like cells obtained after differentiation from mesenchymal stromal cells (Bornstein et al, 2012).

The term is used also to refer to such multicellular aggregates formed by hepatocellular carcinoma cells, which are thought to contain cancer stem cells (Martin-Padura et al, 2012; Lee et al, 2011). See also: sphere forming assay.

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