RT-PCR quantitation of cytokines

PCR is an in vitro technique allowing the selective enrichment of minute amounts of DNA regions of defined lengths and defined sequences from a heterogeneous population of other DNA molecules often present only in a single cell.

This technique exploits some of the properties of DNA polymerase enzymes, which require a short double-stranded region (primer) to produce a double-stranded molecule from single-stranded starting material.

In principle the DNA containing the region to be amplified is made single-stranded by heating. It is then mixed with two chemically synthesized strand-specific oligonucleotides (PCR ... ... ... ...
 
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Entry last modified: March 2002