NRIF3 |
NRL |
cerebral dopamine neurotrophic factor |
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(NRK = normal rat kidney) A cell line established from a mixed population of rat kidney and fibroblasts cells. The cultivation of these cells in serum-free media requires EGF, insulin, and retinoic acid.
The cells are used to assay transforming growth factors (see: TGF). The TGF-beta assay is based on the stimulation of colony formation of NRK cells grown in soft agar (see also: Colony formation assay) in the presence of EGF, which overcomes contact inhibition, and 10 % serum (ED50 = 100 pg/mL).
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