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Cytokine flow cytometry

Flow cytometry offers a way to detect cytokines at the single cell level by means of fixation and permeabilization of cells with subsequent specific intracellular staining with the use of labelled monoclonal anti-cytokine antibodies. The technique also allows determination of cytokine production phenotypes by the simultaneous detection of two or more cytokines within a single cell. This is particularly useful, for example, for the identification of distinct T-helper cell subsets of lymphocytes (Th1 cells, Th2 cells) that differ in their cytokine production profiles. A survey of the kinetics of in situ cytokine production can be carried out also. The technique is useful also to correlate ... ... ... ... ... Subscribe to continue reading! ... ...

 

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