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Cell blot assay

This assay utilizes biologically active growth factors retained on nitrocellulose or polyvinylidene difluoride membranes after transfer from polyacrylamide gels. Cells cultured on blotted lanes can respond to growth factors and cytokines. Alternatively, cells are cultured on pieces of membrane, on which their secretory products are adsorbed and immunostained.

The cell blot assay has been adapted to detect CNTF (ciliary neurotrophic factor), FGF, and NGF.

For other detection systems see also: cytokine assays. See also: bioassays.

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