3D Cell Death Assay

abbr. 3DELTA. This cell death assay has been described by Demuynck R et al (2020). The method employs cell aggregates known as spheroids (tumor spheroids in this case). These cell aggregates are permeabilised with Triton X-100. Sytox dyes, which penetrate all cells in the spheroids, are used as a cell death marker. In the presence of suitable inhibitors (zVAD-fmk for apoptosis, necrostatin-1 for necroptosis, ferrostatin-1 for ferroptosis), the assay, which does not require the disaggregation of the cell aggregates, allows detection of different cell death ... ... ... ...
 
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Entry last modified: October 2022